Indian Journal of Dairy Science
NOVEMBER-DECEMBER 2012, VOL. 65, No. 6
Scanning Electron Microscopy and phagocytic
activity of blood and milk neutrophils isolated
from early lactating buffaloes
Arpita Mohapatra, D.K.
Swain, Sashipal, M.M.K. Pathan, Mandheer Kaur, Shakti Ranjan
Panigrahy, Suman Kapila, Rajeev Kapila, Shiv Prasad, A.K.
Mohanty and A.K. Dang
considered as the first line of cellular defence in the
mammary gland. Neutrophils are more active in blood but once
they cross the mammary barrier, their activity gets reduced
significantly. This reduction in the neutrophilic activity
makes the mammary gland more susceptible to infections. The
decreased activities of the neutrophils are associated with
altered structural and functional integrity of the
neutrophils. No information is available regarding the
alterations in surface architecture of the blood and milk
neutrophils in early lactating buffaloes. The current study
was designed in Murrah buffalo cows during early lactation
to evaluate the structural alterations in both blood and
milk neutrophils. Scanning electron microscopy was carried
out to evaluate the surface dynamics of the neutrophils.
Phagocytic activity was also estimated to correlate the
changes in ultrastructure with activity of neutrophils. The
study concluded that the surface of blood neutrophils were
more ruffled as compared to that of milk neutrophils.
Reduced ruffledness of the membrane of milk neutrophils
indicated a decreased neutrophilic activity and hence the
chances of increased infection of mammary gland during early